Bioremediation of hexavalent chromium by an indigenous bacterium Bacillus cereus S10C1: optimization study using two level full factorial experimental design

The present study is focussed on chromium removal by indigenous chromium-resistant microorganisms isolated from soil and sludge samples of an industrial site. optimal bioremediation conditions, namely temperature, hexavalent (Cr(VI)) initial concentration, pH time were determined using a full factorial design at two levels. results showed that the 54 bacterial strains, S10C1 identified as Bacillus cereus 4080 LBK (NCBI:txid1396) was most efficient strain in removing Cr(VI). analysis variance (ANOVA) indicated pH, Cr(VI) interaction terms (T°–pH), (T°–time) (Cr(VI) concentration–pH), have significant influence yield. Moreover, prescribed first-order regression model fitted well to experimental data with high coefficient determination (R 2 =0.969). According response optimizer Design-Expert software, optimum conditions for biodegradation were: 3, temperature 55 °C, concentration 0.5 mM contact 20 hours. Under these percentage found be about 92.9% which close predicted value statistical (94.4%).